Simultaneous separation and validation of benzoic acid compounds by capillary electrophoresis

Gao Suya; Li Ting; Li Hua

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Simultaneous separation and validation of benzoic acid compounds by capillary electrophoresis

Gao Suya 1,Li Ting 2,Li Hua 2 * #

1.Department of Pharmaceutical Science, Xi’an Medical University;Institute of Analytical Science, Northwest University

2.Institute of Analytical Science, Northwest University

*Correspondence author

#Submitted by

Subject:

Funding: 教育部博士点基金（No.20060697009）

Opened online:19 March 2010

Accepted by: none

Citation: Gao Suya ,Li Ting ,Li Hua .Simultaneous separation and validation of benzoic acid compounds by capillary electrophoresis[OL]. [19 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40889

A simple and inexpensive capillary electrophoresis (CE) was applied to separate six benzoic acid compounds with similar structure. The investigation was carried out by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC). To avoid a time-consuming and tedious procedure, orthogonal experimental design for separation experiments was applied to find the optimal conditions in terms of the resolution and analytical time. The best conditions for separation were obtained using a 20 mM borax and 30 mM sodium dodecyl sulfate (SDS) buffer (pH9.8) containing 2 mM β-CD and 4% methanol (volume fraction). Online UV detection was performed at 250 nm. A voltage of 16 kV was applied and the temperature was controlled at 21℃. Gravity injection was performed for 5 s. The method was validated for the quantification of benzoic acid and salicylic acid in Zuguangsan powder, a traditional Chinese patent medicine. The separation and determination were satisfactory, quick and reliable.

Keywords:Capillary electrophoresis (CE);Separation;Orthogonal experimental design;Benzoic acid

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