A New Hindlimb Model for Inducing Extensor Digitorum Longus Atrophy in Rats: Tail suspension-immobilization

Du Fangying; Wang Jun; Gao Yunfang; Wang Huiping; Wang Qi; Jiang Shanfeng; Nandu Goswami

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A New Hindlimb Model for Inducing Extensor Digitorum Longus Atrophy in Rats: Tail suspension-immobilization

Du Fangying 1,Wang Jun 1,Gao Yunfang 1 * #,Wang Huiping 1,Wang Qi 1,Jiang Shanfeng 1,Nandu Goswami 2

1.College of Life Sciences, Northwest University

2.Institute of Physiology, Center of Physiological Medicine, Medical University Graz

*Correspondence author

#Submitted by

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Funding: Key Laboratory foundation of Resource Biology and Biotechnology in Western China （No.KH09026）, Key Laboratory foundation of Bio-resources in Shaanxi （No.09JS051）, National Nature Science Foundation of China （No.No. 30770273）

Opened online:10 August 2010

Accepted by: none

Citation: Du Fangying,Wang Jun,Gao Yunfang.A New Hindlimb Model for Inducing Extensor Digitorum Longus Atrophy in Rats: Tail suspension-immobilization[OL]. [10 August 2010] http://en.paper.edu.cn/en_releasepaper/content/4380838

IObjectives: To examine the effects of a tail suspension-immobilization (TS+Im) and tail suspension only (TS) model on the morphous and fibre type distribution of the extensor digitorum longus (EDL) of rats. Quicker onset of disuse atrophy would reduce the amount of time the animals have to be subjected to tail suspension. Methods: 48 female Sprague-Dawley rats were randomly divided into eight groups: the new hindlimb disuse model modeled group (with tail suspended and one hindlimb fixed by plaster-casting) and the control group. The EDL in the body matched groups were assessed using 1 week (wk), 2wk, 3wk, 4wk, 5wk, 6wk and 7wk of modeling (with tail suspended and one hindlimb fixed by plaster-casting). The frozen sections of the EDL were prepared, and the types of muscle fibre classified according to the intensity of their mATPase dyeing through the light microscope. The cross sectional area (CSA) of the single muscle fibres was measured using the software Motic Images Plus 2.0. Results: The CSA of EDL in rats decreased gradually after TS and TS+Im. The decrease of EDL’s CSA caused by TS+Im was much obvious than that caused by TS only. TS lead to the transition of type IIB fibres to type I and IIA fibres while TS+Im led to type I and IIA fibres getting converted to type IIB fibres. Conclusions: When compared to TS only, TS+Im cause faster and much more reduction of the EDL‘s CSA in rats. TS+Im can also lead to a shift from oxidative to glycolytic metabolism of the EDL muscle fibre. The tail suspension-immobilization is a new and more effective hindlimb disuse model for the EDL.

Keywords:zoology; tail suspension; immobilization; disuse atrophy; EDL

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