| |
| In this study, the effects of different forms and concentrations of Se on the regulation of glutathione peroxidase (GPx) activity and mRNA levels of phospholipid hydroperoxide GPx (GPx4) and type I deiodinase (D1) in chicken hepatocytes were evaluated. Primary cultured chicken hepatocyte monolayers derived from male White Leghorn chickens (aged 30-40 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 μmol/l of Se from DL-selenomethionine (Se-Met), Kappa-selenocarrageenan (Se-Car) or sodium selenite (Na2SeO3). Compared with controls, significant increases of GPx activity were observed in all hepatocytes treated with Se except for the hepatocytes treated with 5 μmol/l of Na2SeO3, with maximal effects at 2 μmol/l of Se-Met or Se-Car and 1.5 μmol/l of Na2SeO3, respectively. Significant decreases of GPx4 mRNA were observed in all hepatocytes treated with Se (vs. control). D1 mRNA was significantly increased in all groups treated with Se (vs. control), with maximal effects at 1.5 μmol/l of Se-Met and 0.5 μmol/l of Se-Car or Na2SeO3, respectively. Se-Met at doses of 1.5-5 μmol/l had greater effect on D1 mRNA than Se-Car and Na2SeO3 at equivalent doses. After resulting in the maximal effect, higher Se supplementation led to a dose-dependent reduction of GPx activity and D1 mRNA in all hepatocytes treated with Se. These results suggest that in chicken hepatocytes, the regulations of GPx and D1 of by different forms and concentrations of Se vary. |
|
| Keywords:Se; GPx activity; GPx4 mRNA; D1 mRNA |
|
