Improvement of Loading Behavior of Myoglobin into Weak Polyelectrolyte Layer-by-Layer Films by Acid Treatment: Electrochemical and Electrocatalytical Studies

Sun Peng; Hu Naifei; Liu Hongyun

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Improvement of Loading Behavior of Myoglobin into Weak Polyelectrolyte Layer-by-Layer Films by Acid Treatment: Electrochemical and Electrocatalytical Studies

Sun Peng 1,Hu Naifei 2,Liu Hongyun 2 * #

1.Department of Chemistry, Beijing Normal University

2.Department of Chemistry, Beijing Normal University, Beijing 100875

*Correspondence author

#Submitted by

Subject:

Funding: National Natural Science Foundation of China （No.NSFC, No. 20975015）, Doctoral Program of Higher Education of China （No.Project for Junior Faculty Members, No. 20070027021）

Opened online:13 January 2011

Accepted by: none

Citation: Sun Peng,Hu Naifei,Liu Hongyun.Improvement of Loading Behavior of Myoglobin into Weak Polyelectrolyte Layer-by-Layer Films by Acid Treatment: Electrochemical and Electrocatalytical Studies[OL]. [13 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4404807

The treatment of {PAH/PAA}n layer-by-layer films with pH 2.5 solutions has a great influence on loading of myoglobin (Mb) from its pH 5.0 buffers into the films, where PAH = poly(allylamine hydrochloride) and PAA = poly(acrylic acid). Without acid treatment, the {PAH/PAA}n films assembled on pyrolytic graphite electrodes are difficult to load or "absorb" Mb from its solution into the films, and the CV response of {PAH/PAA}n-Mb films is very small. However, after {PAH/PAA}n films are immersed in pH 2.5 buffers for only several minutes, the films can load much larger amounts of Mb from its solutions, and the corresponding {PAH/PAA}n-Mb films show good and nearly reversible cyclic voltammetric response with large peak currents. The direct electrochemistry of {PAH/PAA}n-Mb films can also be used to electrochemically catalyze the reduction of oxygen and hydrogen peroxide in solution. Electrochemical impedance spectroscopy and scanning electron microscopy results show that the permeability and porosity of {PAH/PAA}n films are significantly improved after the acid treatment, and thus beneficial for the loading of Mb from its solution.

Keywords:Electrochemical analysis; weak polyelectrolytes; layer-by-layer assembly; loading beavior; direct electrochemistry; myoglobin

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