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Molecular analysis of quizalofop-resistant Echinochloa crusgalli reveals a Leu-1781-Ile mutation
HUAN Zhibo #,WANG Jinxin *
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University
*Correspondence author
#Submitted by
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Funding: Ph.D. Programs Foundation of Ministry of Education of China （No.No. 20093702110003）
Opened online:14 March 2011
Accepted by: none
Citation: HUAN Zhibo,WANG Jinxin.Molecular analysis of quizalofop-resistant Echinochloa crusgalli reveals a Leu-1781-Ile mutation[OL]. [14 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4414727
 
 
A resistant Echinochloa crusgalli (barnyard grass) population which survived after repeated application of quizalofop in China was collected. After self-pollination and outcross, a homozygous germplasm (RR) was obtained. The resistant germplasm (RR) was found highly resistant to quizalofop compared with the sensitive germplasm (SS) with the resistance ratio 86.1. In order to determine the mechanism of this resistance, the chloroplastic ACCase cDNA from RR and SS were cloned. After compared the deduced amino acid sequences, a single isoleucine-leucine substitution at position 1781 (position number standardized to Alopecurus myosuroides (black grass)) was found. This mutation was identical with the formerly reported results in R population of other weed species. In the allele-specific PCR assay, we found RR and SS were all homozygous. All the copies in RR were Leu1781 and SS were Ile1781.?????
Keywords:weed control; barnyard grass; quizalofop;gene mutation; Allele-specific PCR.
 
 
 

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