Purification and Characterization of a novel β-galactosidase from the newly isolated strain Erwinia rhapontici E602

JIANG Yike; XIA Yu

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Purification and Characterization of a novel β-galactosidase from the newly isolated strain Erwinia rhapontici E602

JIANG Yike 1,XIA Yu 2 * #

1.School of Food Science and Technology, Jiangnan University

2.School of Food Science and Technology, Jiangnan University, Wuxi 214122

*Correspondence author

#Submitted by

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Funding: National Research Foundation for the Doctoral Program of Higher Education of China （No.No. 200802951022）, National Natural Science Foundation of China （No.No. 31000752）, Science and Nature Foundation in Jiangsu Province （No. No. BK2008103)）

Opened online:30 June 2011

Accepted by: none

Citation: JIANG Yike,XIA Yu.Purification and Characterization of a novel β-galactosidase from the newly isolated strain Erwinia rhapontici E602[OL]. [30 June 2011] http://en.paper.edu.cn/en_releasepaper/content/4433503

A β-galactosidase producing bacterial strain E602 was isolated from frozen soil and identified to be Erwinia rhapontici. This strain was cultured at 26℃ with the induction of lactose for production of the β-galactosidase. This enzyme was then purified with ammonium sulfate fractionation, ion exchange and gel filtration chromatography for further studies of the enzymatic characteristics. The purified enzyme had a molecular weight of about 100 kDa. The optimum reaction temperature and pH of this enzyme was 40℃ and pH 7.0 respectively, indicating that this enzyme was a mesophilic neutral β-galactosidase. However, the enzyme retained about 15% activity at 10℃, which also suggested cold-adaptive property of this enzyme. Kinetics of this enzyme was studied and the Km and Vmax of this enzyme was 0.21 mmol/L and 263.16 μmol/mg min-1. Metal ions such as Li+, K+, Mg2+ and Zn2+ can activate the enzymatic activity obviously, while Cu2+ and Fe2+ inhibited the activity. The lactose hydrolysis in milk had been also investigated and results suggested that this enzyme may be suitable for milk processing.

Keywords:β-Galactosidase; Erwinia rhapontici; Isolation and Purification; Enzymatic Characteristics

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