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| Human fetal liver aged 22 weeks of gestation (HFL22w) consists of hepatic parenchymal cells and hematopoietic stem/progenitor cells, not only has liver functions, but also has hematopoietic functions, and is a turning point between immigration and emigration of hematopoietic system. In this article, the EST data were sequenced, and clustered by blasting against five human transcriptome databases. Then after EST assembly and gene identification, the known genes were classified by GO, and the unknown genes were analyzed by Pfam and ScanProsite to predicted their functions. This database is comprised of six parts: introduction, methods, significance, known genes, unknown genes and download. The results show that: (1) 12368 high-quality ESTs were obtained in 20282 sequences. (2) In 12368 ESTs, 8097 ESTs were matched to 2483 known genes, and 4271 ESTs were identified as 2416 genes that exhibited no significant homology to known genes. (3) In 2416 genes, 1379 genes were identified as fully new sequences. (4) Through GO classification, 6 cell migration genes and 6 hemopoiesis genes were confirmed. (5) Prediction of gene function had enabled us to obtain 277 profiles. Among them, there are five categories distributed in more than 10 genes. (6) We have built the world's biggest EST database on HFL22w. This database on HFL22w will help to understand hemopoiesis and cell migration mechanism, and promote future study on HFL22w for all interested researcher. ESThfl22w can be freely accessed at http://www.ikaixue.com/chen. |
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| Keywords:human fetal liver; expression sequence tags; database |
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