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Identification of HrpX Regulon Genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique
LI Yurong 1,XIAO Youlun 2,ZOU Lifang 3,ZOU Huasong 3,CHEN Gongyou 3 *
1.Department of Agriculture and Biology, Shanghai Jiao Tong University, ShangHai 200240
2.Institute of Plant Protection, Hunan Academy of Agriculture Sciences, 410425
3.Department of Agriculture and Biology, Shanghai Jiao Tong University, 200240
*Correspondence author
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Funding: the Ph.D. Programs Foundation of Ministry of Education of China （No.No. 20100073110045）, the Natural Science Foundation of China （No.No. 31071656）, the State Key Basic Research and Development Project of China （No.No. 2012CB114000）
Opened online: 9 November 2011
Accepted by: none
Citation: LI Yurong,XIAO Youlun,ZOU Lifang.Identification of HrpX Regulon Genes in Xanthomonas oryzae pv. oryzicola using a GFP visualization technique[OL]. [ 9 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4447535
 
 
Xanthomonas oryzae pv. oryzicola is the causal agent of bacterial leaf streak in rice and injects repertoires of T3S effectors (T3SEs), which are normally regulated by a global regulator HrpX, into plant cells to suppress plant innate immunity for disease development. To establish a visualization technique to identify HrpX regulon genes in this pathogen, we chose six known or unknown T3SE genes of X. oryzae pv. oryzicola (strain RS105) as the targets in this report. The promoters of these candidates, whether or not containing a PIP-box (plant-inducible promoter), were fused with the gfp (green fluorescent protein) reporter gene, and the gfp reporters were introduced into the wild-type RS105 and the hrpX mutant RΔhrpX. GFP expression in the wild-type strain could be observed under fluorescence microscopy, but fluorescence was not observed in the hrpX mutant. Consequently, transcription and secretion detection demonstrated that these HrpX regulon members were repressed when hrpX was mutated and were not secreted into the hrp-inducing medium XOM3 because of a mutation in hrcV (T3SS deficient). These data suggest that the gfp reporter system is a feasible visualization tool for identification of HrpX regulon genes and discovery of new T3SEs in the X. oryzae pv. oryzicola-rice pathosystem.
Keywords:X. oryzae pv. oryzicola; HrpX-regulon gene; gfp; PIP-box
 
 
 

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