Optimization of Agrobacterium-mediated transformation system for soybean callus

Liu Dong; Chang Dasong; Liu Sheng; Wang Dan; Wang Ningning

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Optimization of Agrobacterium-mediated transformation system for soybean callus

Liu Dong,Chang Dasong,Liu Sheng,Wang Dan,Wang Ningning * #

Department of Plant Biology and Ecology, Nankai University, Tianjin 300071, China

*Correspondence author

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Funding: The Major Technological Program on Cultivation of New Varieties of Genetically Modified Organisms （No.Nos 2008ZX08004-005 and 2009ZX08009-133B) and Specialized Research Fund for the Doctoral Program of Higher Education of China）

Opened online: 9 January 2012

Accepted by: none

Citation: Liu Dong,Chang Dasong,Liu Sheng.Optimization of Agrobacterium-mediated transformation system for soybean callus[OL]. [ 9 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4458595

This report described a simple and efficient method of Agrobacterium-mediated transformation for obtaining transgenic callus tissues of soybean [Glycine max (L.) Merr.]. Hypocotyl segment explants of soybean were used as the starting material. To improve transformation efficiencies, several factors such as inoculation time, co-cultivation period and addition of antioxidants in co-cultivation medium (CCM) were examined. Callus tissues were induced at both the acropetal and basipetal ends of explants after about 2 weeks of culture on callus induction medium (CIM) containing 0.5 mgoL-1 6-Benzylaminopurine (6-BA) and 2.0 mgoL-1 2,4-Dichlorophenoxyacetic acid (2,4-D). The average transformation efficiency reached up to 87.7% based on GUS detection.

Keywords:soybean; hypocotyl segment; Agrobacterium tumefaciens; callus tissues; transformation

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