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| In order to characterize the effect of 2, 6-dinitrotoluene (2, 6-DNT) on gene expression in the liver of common carp (Cyprinus carpio), fish was exposed via water for 15 days at concentrations of 0.195 and 1.95 mg/l 2,6-DNT in semi-static bioassay. Liver was selected as target tissue. Custom microarrays were constructed from cDNA libraries obtained with Suppression Subtractive Hybridization-Polymerase Chain Reaction (SSH-PCR) experiments. Microarray data revealed that the expression of several genes in the liver was influenced by 2,6-DNT exposure and real-time PCR was used to confirm these gene expression changes. The affected genes were mainly involved in energy metabolism, biotransformation and stress response. The pattern of EROD, GST，Na+/K+-ATPase, and GK enzyme activity in liver of common carp may have a close relationship with the gene expression and stress response to 2, 6-DNT. Furthermore, the RCF, HSI, and the available glycogen reserves of the exposed fish were significantly lower after 15 days of exposure than in the control fish. Through principal component analysis, effects by 2, 6-DNT-stress in each test group were distinguished. In this study, indications of a trade-off between the metabolic cost of toxicant exposure and processes vital to the survival of the organism were seen on the molecular level as well as on higher levels of biological organization. |
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| Keywords:Environmental science; Common carp; 2, 6-DNT; Gene expression; Enzyme activity; Energy reserves |
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