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Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua
Liang Yuerong 1 #,Lin Chen 2,Wang Xunlei 2,Ye Jianhui 2,Lu Jianliang 2 *
1.Zhejiang University Tea Research Institute, Hangzhou 310058
2.Zhejiang University Tea Research Institute, Hangzhou 310058
*Correspondence author
#Submitted by
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Funding: This work was supported by Doctoral Foundation of Ministry of Education in China （No.No.200803350093）
Opened online: 8 February 2012
Accepted by: none
Citation: Liang Yuerong,Lin Chen,Wang Xunlei.Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua[OL]. [ 8 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4463882
 
 
Ectropis obliqua is a major tea pest and chitin synthase (CHS) plays a key role in the pest growth and development. A 192 bp conserved domain from E. obliqua CHS gene was cloned and it was used to construct recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) with double-stranded RNA interference (dsRNAi) method. The recombined AcMNPV virus could propagate in host cells sf9. Injection test showed that the virus efficacy of the recombined AcMNPV on E. obliqua larvae was significantly enhanced. It is considered that the CHS dsRNAi mediated by the Nuclear polyhedrosis virus will be interesting for development of alternative bio-pesticide to control the tea pest E. obliqua.
Keywords:Tea science; Chitin synthase; Baculovirus; double-stranded RNA interference; Ectropis obliqua
 
 
 

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