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Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus)
WU Haitao 1 #,KE Zhen 2,ZHANG Jianrong 3,XU Xinzhou 2,LIU Meng 2,ZHANG Shuangquan 2 *
1.Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, NanJing 210046
2.Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210046, PR China
3.Hongshan Forest Zoo, Nanjing 210028, PR China
*Correspondence author
#Submitted by
Subject:
Funding: The Research Fund for the Doctoral Program of Higher Education （No.No. 200803160002）, The Priority Academic Program Development of Jiangsu Higher Education Institutions（No.No.30271093）
Opened online:21 February 2012
Accepted by: none
Citation: WU Haitao,KE Zhen,ZHANG Jianrong.Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus)[OL]. [21 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465365
 
 
B-cell survival depends on signals induced by B-cell activating factor (BAFF) binding to its receptor (BAFF-R). In mice, mutations in BAFF or BAFF-R cause B-cell lymphopenia and antibody deficiency. However, the information about horse is poorly understood. Here, We report the identification and analysis of a novel horse TNF receptor related to B-cell suivival, BAFF-R. The horse BAFF-R gene spans 1444 bp, including three exons and located on chromosome 28. The horse BAFF-R protein is composed of 183 aa, with the calculated molecular weight 19 kDa and isoelectric point 6.12. As a type Ⅲ transmembrane protein, its extracellular domain has one cysteine-rich domain (CRD) containing four cysteine residues, and cytoplasmic domain exists the TRAF3 binding domain. The genetic relationship between horse and dog is the closest by the phylogenetic analysis of BAFF-R amino acids. Moreover, recombinant BAFF-R-Fc protein not only binds to B-cell, but also inhibits B-cell survival induced by BAFF in vitro. These studies provide the basis for investigation on its roles in regulating B lymphocyte survival and related immune diseases in horse.
Keywords:horse; BAFF-R; B-cell survival
 
 
 

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