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ac109 is required for the nucleocapsid assembly of Autographa californica multiple nucleopolyhedrovirus
Lin Lin 1,Riqiang Deng 2,Jinwen Wang 2,Jianhao Ke 2,Hongkai Wu 2 #,Xunzhang Wang 2 *
1.State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, GuangZhou 510275
2.State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 510275 Guangzhou, China
*Correspondence author
#Submitted by
Subject:
Funding: the State Key Laboratory for Biocontrol under grant(No.No. SKLBC-08-B-01), the Natural Science Foundation of Guangdong Province Grant(No.No.4203388), Research Fund for the Doctoral Program of Higher Education of China grant(No.No.200805580033)
Opened online:21 March 2012
Accepted by: none
Citation: Lin Lin,Riqiang Deng,Jinwen Wang.ac109 is required for the nucleocapsid assembly of Autographa californica multiple nucleopolyhedrovirus[OL]. [21 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471364
 
 
ORF109 (Ac109) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved gene in all sequenced baculovirus genomes, but its function is not known. This paper describes generation of an ac109 knockout virus (Ac-ac109-KO-GP) and analyses of the influence of ac109 deletion on the virus replication in Sf-9 cells so as to investigate the role of ac109 in the viral life cycle. Results revealed that budded virus (BV) yields and occlusion body synthesis were completely blocked in cells infected with the mutant virus. Electron microscopy demonstrated that ac109 deletion blocked nucleocapsid formation, though infection was initiated and electron-dense bodies associated with the virogenic stroma appeared. The mutant phenotype was rescued by an ac109 rescue virus. On the other hand, real-time PCR analysis indicated that ac109 is not required for viral DNA replication. Thus, these results suggested that ac109 plays an important role in AcMNPV nucleocapsid formation.
Keywords:Baculovirus; ac109; AcMNPV; Nucleocapsid formation
 
 
 

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