Fibroblast  activity on multilayer laminin5 beta3 DNA coating titanium surface

JIANG Qiaohong; ZHAO Shifang; GONG Xue; HE Fuming; YANG Guoli

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Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface

JIANG Qiaohong 1,ZHAO Shifang 2,GONG Xue 3,HE Fuming 4,YANG Guoli 5 *

1.The Affiliated Stomatology Hospital, School of Medical, Zhejiang University, Hangzhou 310006

2.The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.

3.The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.

4.The Affiliated Stomatology Hospital, School of Medical, Zhejiang University, Hangzhou 310006, China.

5.The Affiliated Stomatology Hospital, School of Medical, Zhejiang University, Hangzhou 310006, China.

*Correspondence author

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Funding: none

Opened online: 9 April 2012

Accepted by: none

Citation: JIANG Qiaohong,ZHAO Shifang,GONG Xue.Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface[OL]. [ 9 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4473769

This work was to investigate the fabrication of multilayer gene-functionalized films composed of cationic liposome (Lip) and pEGFP-C1-LAMB3 (pGL) DNA on titanium surfaces and the effect of the gene coatings on NIH3T3 cells. The multilayer gene coatings were fabricated on titanium by layer-by-layer (LbL) assembly technique. Surfaces analysis was carried out by contact angle measurements and X-ray photoelectron spectroscopy (XPS). Formation of LBL films was confirmed by fluorescence microscope. A biological study with NIH3T3 was followed over a 7-day culture period at days 0, 3 and 7 to observe the viability. This study showed polyelectrolyte multilayer films could be successfully deposited. EGFP expression increased with adding layer numbers. NIH3T3 adhered on multilayered DNA coatings displayed significantly higher (P<0.05) cell viability compared to control group both for 3 and 7 days culture. The results indicated the HA/pGL coatings improve the cytocompatibility of native titanium surface and presents a promising platform for delivery of DNA to NIH3T3 cells.

Keywords:Transfection; Gene; Laminin5; Liposome; Titanium

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