Cloning and Functional characterization of three Superoxide Dismutases genes from halophyte Salicornia europaea and Thellungiella halophila

Wu Fan; Mei Yu; Lu Maolong; Juan Li; Wang Rongfu; Wang Xianlei; Sun Qizhen; Jie Zang; Kai Xu; Hu Ji

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Cloning and Functional characterization of three Superoxide Dismutases genes from halophyte Salicornia europaea and Thellungiella halophila

Wu Fan 1 #,Mei Yu 2 *,Lu Maolong 3,Juan Li 3,Wang Rongfu 3,Wang Xianlei 3,Sun Qizhen 2,Jie Zang 2,Kai Xu 2,Hu Ji 2

1.Academy of Life Science, Anhui Agricultural University, HeFei 230036

2.Academy of Life Science, Anhui Agricultural University, Hefei City, 230036

3.Center of Biotechnology, Anhui Agricultural University, Hefei City, 230036

*Correspondence author

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Funding: none

Opened online:25 April 2012

Accepted by: none

Citation: Wu Fan ,Mei Yu,Lu Maolong.Cloning and Functional characterization of three Superoxide Dismutases genes from halophyte Salicornia europaea and Thellungiella halophila[OL]. [25 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4474413

In order to study the halophyte salt-tolerance mechanism, we cloned the manganese (Mn) and Cu/Zn superoxide dismutase (SOD) full-length cDNAs from Salicornia europaea by RACE method for the first time, sequence analysis indicated that the MnSOD gene (GenBank accession number: JQ061158) comprises an open reading frame of 699 bp, encoding 233-amino acid polypeptide with a predicted molecular mass of 25.7 kDa. Correspondingly, the Cu/ZnSOD gene (GenBank accession number: JQ061160) consists of an open reading frame of 684 bp which encodes a protein of 228 amino acids with a predicted molecular mass of 23.3 kDa. The prokaryotic expression vectors pET30-SeMSD, pET30-SeCSD and pET30-ThMSD were constructed, and the target proteins were expressed successfully in BL21 Escherichia coli. Through optimization of the inducing concentration of Isopropyl β-D-Thiogalactopyranoside (IPTG), we tested the salt tolerance of these three superoxide dismutases under 6.5% and 7.5% NaCl, and the results demonstrate that the recombinants BL21 (pET30-SeMSD) and BL21 (pET30-ThMSD) show better tolerance to salinity stress in comparison with the control stain BL21 (pET30), but the recombinant BL21 (pET30-SeCSD) has not displayed increased salt tolerance.

Keywords:Molecular Cloning; Salicornia europaea; SeMSD; SeCSD; ThMSD; Salt tolerance

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