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Effects of truncated mutation of APC on cell-matrix and cell-cell adhesion in kidney epithelial cell lines
LI Wenling 1 #,ZHU Wensi 2,NIU Haibo 2,SONG Li 2,LI Zhuoyu 2 *
1.The Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Institute of Biotechnology, Shanxi University, TaiYuan 030006
2.The Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Institute of Biotechnology, Shanxi University, 030006 Taiyuan, P. R. China
*Correspondence author
#Submitted by
Subject:
Funding: Shanxi scholarship council of China （No.201010）, Special research foundation of the doctoral program of higher education （No.20111401110011）, International collaboration projects （No.2011081058）, National Natural Science Foundation of China （No.No.31040018&8115026）
Opened online:26 April 2012
Accepted by: none
Citation: LI Wenling,ZHU Wensi,NIU Haibo.Effects of truncated mutation of APC on cell-matrix and cell-cell adhesion in kidney epithelial cell lines[OL]. [26 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4475722
 
 
APC is associated with cell adhesion, but the effects of truncted APC and the mechanism are not well defined.To explore the impact of mutant adenomatous polyposis coli (APC) on cell-matrix, cell-cell adhesion and the relative mechanism. Cell-matrix and cell-cell adhesion assay were employed to determine the adhesion level of two stable cell lines MDCK-N2-APC and MDCK-GFP. The truncated APC of N2 fragment, which spans residues 449-781 was studies compared to control cells including GFP alone. The immunofluorescence staining, RT-PCR analysis and Western blotting were applied to check several adhesion molecular which have key roles in cell contacts process. In contract with control, cell-matrix adhesion was averagely increased to180% in N2 cells, whereas, cell-cell adhesion was reduced by about 30%. Our experiments indicated that N2 fragment of APC decreases cell adhesion via enervating E-cadherin expression level. It enhances cell adhesion by means of improving CD29 level respectively. These data suggest that full length APC plays a crucial role in cell-matrix adhesion and cell-cell adhesion. The truncation mutation of APC fragment, N2 restrained in the colon cancer cells, will alter the cell invasion and migration by the influence on cell adhesion and cell- matrix adhesion
Keywords:cell-matrix adhesion; cell-cell adhesion; adenomatous polyposis coli (APC); truncated mutation
 
 
 

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