An oxidoreduction potential shift control strategy for high purity propionic acid production by Propionibacterium freudenreichii CCTCC M207015 with glycerol as sole carbon source

CHEN Fei; FENG Xiaohai; LIANG Jinfeng; XU Hong

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

An oxidoreduction potential shift control strategy for high purity propionic acid production by Propionibacterium freudenreichii CCTCC M207015 with glycerol as sole carbon source

CHEN Fei #,FENG Xiaohai,LIANG Jinfeng,XU Hong *

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 210009

*Correspondence author

#Submitted by

Subject:

Funding: the Research Fund for the Doctoral Program of Higher Education of China （No.No. 20103221110006）, the Graduate Student Innovation Project of Jiangsu Province （No.No. CXLX12_0464）

Opened online:11 June 2012

Accepted by: none

Citation: CHEN Fei,FENG Xiaohai,LIANG Jinfeng.An oxidoreduction potential shift control strategy for high purity propionic acid production by Propionibacterium freudenreichii CCTCC M207015 with glycerol as sole carbon source[OL]. [11 June 2012] http://en.paper.edu.cn/en_releasepaper/content/4480997

The effects of oxidoreduction potential (ORP) regulation on the process of propionic acid production by Propionibacterium freudenreichii CCTCC M207015 have been investigated. Potassium ferricyanide and sodium borohydride were to determine as ORP control agents in serum bottle. In batch fermentation, cell growth, propionic acid and by-products distribution were changed with ORP levels in the range of 0 to 160 mV. Based on these analysis results, an ORP-shift control strategy was proposed: At first 156 h, ORP was controlled at 120 mV to obtain higher cell growth rate and propionic acid formation rate, then it was shifted to 80 mV after 156 h to maintain the higher propionic acid formation rate. By applying this ORP-shift control strategy, the optimal parameters were obtained as follows: the propionic acid concentration 45.99 g/L, productivity 0.192 g/L/h, the proportion of propionic acid to total organic acids 92.26% (w/w) and glycerol conversion efficiency 76.65%. The mechanism of ORP regulation was discussed by the ratio of NADH/NAD and ATP levels. The results suggest that it is possible to redistribute metabolic fluxes by the ORP-shift control strategy, and the strategy could provide a simple and efficient tool to realize high purity propionic acid production using glycerol.

Keywords:fermentation engineering; propionic acid; glycerol; Propionibacterium freudenreichii CCTCC M207015; oxidoreduction potential shift control strategy; energy detection

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	392
Bookmarked	1
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers