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| Apelin, a novel neoropeptide, was identified as the endogenous ligand for APJ receptor. The present study was designed to explore the effect apelin-13 on interdigestive gastric motility in conscious rats. Apelin-13 was synthesized by the solid-phase peptide synthesis method. After recovery from the operation of intracerebroventricular (i.c.v.) cannula implantation, the strain gauge transducers were implanted on the serosal surface of the antrum in Wistar rats. The migrating motor complex (MMC) was instantly recorded by the Power-Lab recording system. The results show that, comparing with control group (5.17±0.93), the frequency of the phase Ⅲ-like activity (c/h) of the 10 nmol apelin-13 group (7.67±1.33) and 30 nmol apelin-13 group (8.58±1.66) were both increased, showing a statistically significant difference (P < 0.01, P < 0.01, respectively). However, frequency of the phase Ⅲ-like activity of 3 nmol apelin-13 group (6.83±0.98) had no significant difference (P > 0.05). Comparing with control group (97.41±16.88), the %motor index (MI) of the 10 nmol apelin-13 group (143.93±26.27) and 30 nmol apelin-13 group (155.09±24.02) were both increased, indicating a statistically obvious difference (P < 0.01, P < 0.01, respectively). However, the %MI of 3 nmol apelin-13 group (120.14±3.00) showed no significant difference (P > 0.05). Our study first indicates that i.c.v. injection of apelin-13 increased the interdigestive antrum MMC in conscious rats, which has an important physiological significance for further study the modulatory effect of apelin-13 on the digestive tract motility. |
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| Keywords:Biochemistry; Apelin-13; Gastric motility; Interdigestive; Rats |
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