Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors

LUO Zuliang; KONG Weijun; QIU Feng; YANG Meihua; LI Qian; WEI Riwei; YANG Xiaoli; QIN Jieping

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Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors

LUO Zuliang 1,KONG Weijun 2 #,QIU Feng 2,YANG Meihua 2 *,LI Qian 2,WEI Riwei 2,YANG Xiaoli 2,QIN Jieping 3

1.Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193

2.Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China

3.Guangxi University of Chinese Medicine, Nanning, 530001, China

*Correspondence author

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Funding: 2011年度高等学校博士学科点专项科研基金（No.20111106110034）, 北京市自然科学基金（No.7112092）

Opened online:20 September 2012

Accepted by: none

Citation: LUO Zuliang,KONG Weijun,QIU Feng.Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors[OL]. [20 September 2012] http://en.paper.edu.cn/en_releasepaper/content/4489444

A simple and sensitive high performance liquid chromatography coupled with photodiode array (HPLC-PDA) method was developed for simultaneous determination of 7 lignans in Justicia procumbens using relative response factors. The chromatographic separation was performed on a Shiseido Capcell Pak C18 column (250 × 4.6 mm id, 5 μm), a gradient elution of acetonitrile/water and a photodiode array detector. The column temperature was maintained at 35 C and the detection wavelength was set at 256 nm. Chinensinaphthol methyl ether was selected as the reference compound for calculating the relative response factors of the lignans. It has shown that the relative response factors (RRFs) for lignans are quite similar at 256 nm of detection under different analytical conditions (different columns and different HPLC instruments). Using RRFs, no lignan is needed as a reference standard, making the method ideal for rapid, routine analysis, especially for those laboratories where lignans standards are not readily available. An economic and practicable HPLC method using RRFs was established for the determination of seven lignans in J. procumbens. This method not only can determine multiple indexes in Chinese materia medica (CMM) simultaneously, but also resolve the problem of lacking of chemical standards. It will be a good quality evaluation method and pattern for CMM.

Keywords:HPLC-PDA; Relative response factors; J. procumbens; Lignans

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