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A Tourist-like MITE insertion in the upstream region of the BnFLC.A10 gene is associated with vernalization requirement in rapeseed (Brassica napus L.)
Hou Jinna 1,Long Yan 2,Zou Xiaoxiao 2,Wang Jing 2,Meng Jinling 2 *
1.National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, WuHan 430070
2.National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070
*Correspondence author
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Funding: Specialized Research Fund for the Doctoral Program of Higher Education（New Teachers)（No.20090146120019）, National Natural Science Foundation of China （No.No. 30900788）
Opened online:16 November 2012
Accepted by: none
Citation: Hou Jinna,Long Yan,Zou Xiaoxiao.A Tourist-like MITE insertion in the upstream region of the BnFLC.A10 gene is associated with vernalization requirement in rapeseed (Brassica napus L.)[OL]. [16 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4494914
 
 
In this paper, we fine-mapped the spring environment specific quantitative trait locus (QTL) for flowering time, qFT10-4，in a doubled haploid (DH) mapping population of rapeseed derived from a cross between Tapidor (winter-type) and Ningyou7 (semi-winter) and delimited the qFT10-4 to an 80-kb region on chromosome A10 of B. napus. The BnFLC.A10 gene, an ortholog of FLOWERING LOCUS C (FLC) in Arabidopsis, was cloned from the QTL. We identified 12 polymorphic sites between BnFLC.A10 parental alleles of the TN-DH population in the upstream region and in intron 1. Expression of both BnFLC.A10 alleles decreased during vernalization, but decreased more slowly in the winter parent Tapidor. Haplotyping and association analysis showed that one of the polymorphic sites upstream of BnFLC.A10 is strongly associated with the vernalization requirement of rapeseed (r2 = 0.93, χ2 = 0.50). This polymorphic site is derived from a Tourist-like miniature inverted-repeat transposable element (MITE) insertion/deletion in the upstream region of BnFLC.A10. The MITE sequence was not present in the BnFLC.A10 gene in spring-type rapeseed, nor in ancestral 'A' genome species B. rapa genotypes. Our results suggest that the insertion may have occurred in winter rapeseed after B. napus speciation.
Keywords:Rapeseed; Flowering time; Vernalization
 
 
 

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