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Effects of over-expression of allene oxide cyclase on camptothecin production by cell cultures of Camptotheca acuminata
PI Yan 1 *,JIANG Keji 2,LIN Juan 3,CAO Ying 3,WANG Qian 3,YANG Ji 3
1.State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433
2.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
3.State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, P. R. China
*Correspondence author
#Submitted by
Subject:
Funding: Research Fund for the Doctoral Program of Higher Education of China(No.No. 20090071120040), National Basic Research Program of China(No.No. 2007CB108805), Shanghai Science and Technology Committee(No.No. 08391911800)
Opened online:27 December 2012
Accepted by: none
Citation: PI Yan,JIANG Keji,LIN Juan.Effects of over-expression of allene oxide cyclase on camptothecin production by cell cultures of Camptotheca acuminata[OL]. [27 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4506263
 
 
Camptothecin (CPT) is an anticancer and antiviral monoterpene-derived indole alkaloid which can be induced by plant hormone, jasmonates. To improve the production of the pharmaceuticals, the jasmonate biosynthesis related gene allene oxide cyclase from Camptotheca acuminate was transferred back into Camptotheca acuminate using the method of Agrobacterium-mediated genetic transformation and over expressed. The results of HPLC analysis indicated that the camptothecin content of transgenic callus was higher than that of non-transgenic callus. The highest camptothecin content in transgenic callus was 3.9310 mg/g DW. However, camptothecin content in both transgenic and non-transgenic calli significantly decreased after further extrinsic MeJA's induction, whereas the content of CPT in transgenic callus was still higher than in non-transgenic one. All the results indicated that endogenic jasmonate's accumulation may be promoted after allene oxide cyclase gene was transformed into Camptotheca acuminate and over expressed. In this way, jasmonates can affect second metabolism pathway genes' expression and then the camptothecin content was improved. Inexpectantly, the mechanism of extrinsic jasmonate to secondary metabolism of Camptotheca acuminate was different from that of endogenic jasmonates.
Keywords:Agrobacterium tumefaciens; Allene oxide cyclase; Camptotheca acuminata; Camptothecin; Methyl-jasmonates; Transformation
 
 
 

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