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Evidence of chilling-induced programmed cell death in cucumber fruit
Chen Xiaohong 1 #,Nie Peng 2,Mao Linchun 3 * #
1.Institute of Postharvest Engineering of Agricultural Products, College of Biosystem Engineering & Food Science, Zhejiang University, HangZhou 310058
2.Institute of Insect Science, College of Agricultural Science, Zhejiang University, Hangzhou 310058, China
3.Institute of Postharvest Engineering of Agricultural Products, College of Biosystem Engineering & Food Science, Zhejiang University, Hangzhou 310058, China
*Correspondence author
#Submitted by
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Funding: none
Opened online:27 December 2012
Accepted by: none
Citation: Chen Xiaohong,Nie Peng,Mao Linchun.Evidence of chilling-induced programmed cell death in cucumber fruit[OL]. [27 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4506670
 
 
Cucumber fruit (Cucumis sativus L. cv. Zhexiu-1) were stored at 2℃ for 3 d, 6 d, 9 d and subsequently reconditioned at 20℃ for 2 d, respectively. Chilling injury (CI) was greatly aggravated upon reconditioning after chilling. Cell death occurred synchronously with CI acceleration. The hallmark of programmed cell death (PCD), DNA laddering, was clearly visualized by genome gel electrophoretic analysis upon reconditioning after chilling for 9 d with ethylene burst. Further evidence of nuclear DNA nicking was confirmed by the in situ TdT-mediated dUTP nick end labelling (TUNEL). In addition, chromatin condensation and nucleus distortion were observed by nuclear staining of DAPI. Results suggested that cell death arisen with the occurrence of chilling injury in cucumber fruit upon reconditioning after cold storage had the features of PCD process which may be mainly attributed to ethylene.
Keywords:postharvest biology; programmed cell death; chilling injury; cucumber
 
 
 

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