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Towards A new molecular tool for transgenic plants: Control of protein biosynthesis by a seed-specific promoter
YANG Yuening 1,CHEN Moxian 2,ZHENG Shuxiao 2,YAO Yao 3,XU Chao 3,WANG Yin 3,LIU Jiesheng 3,CHYE Meelen 2,YANG Weidong 3,LI Hongye 3 * #
1.Department of Biotechnology, Jinan University, Guangzhou 510632
2.School of Biological Sciences, The University of Hong Kong, Hong Kong, China
3.Department of Biotechnology, Jinan University, Guangzhou, 510632, China
*Correspondence author
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Funding: SRFDP （No.No. 9151063101000001）, Guangdong Natural Science Foundation （No.No.20094401120010）
Opened online:10 January 2013
Accepted by: none
Citation: YANG Yuening,CHEN Moxian,ZHENG Shuxiao.Towards A new molecular tool for transgenic plants: Control of protein biosynthesis by a seed-specific promoter[OL]. [10 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4510784
 
 
[Background] Plants have been proven to be promising bioreactors in the production of many heterologous proteins including those in the biopharmaceutical industry. However, the efficiency of a potential plant bioreactor is impaired by the limitation in available promoters to drive foreign gene expression. Hence, the discovery of novel promoters is urgently needed. Seeds are ideal for protein storage and proteins can be kept as a stable form for several months. Thus a strong seed-specific promoter is a powerful tool in directing the expression and accumulation of target proteins in the seed. [Results] A 784-bp 5'-flanking sequence of the mung bean seed storage protein 8S globulin α' subunit gene was isolated by genome walking. When the sequence was analyzed with bioinformatics tools, numerous putative cis-elements were identified. The green fluorescent protein (GFP) reporter driven from this promoter was transiently expressed in protoplasts derived from mung bean cotyledons. Finally, transgenic Arabidopsis plants expressing the β-glucuronidase (GUS) reporter gene driven from the 8S globulin alpha' promoter were generated. GUS expression in transgenic seeds was observed by histochemical and quantitative GUS assays, confirming seed expression and high transcriptional activity. This study has identified the mung bean 8S α' promoter having potential for directing expression in seeds for bioreactor application.
Keywords:Seed; promoter; genome walking; mung bean.
 
 
 

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