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Isolation and Characterization of Total N-linked Glycans from Glycoproteins by Ultrafiltration Units and Mass Spectrometry
YANG Ganglong,MA Tianran,LI Zheng * #
Life Science School, Northwest University,Xi'An, 710069
*Correspondence author
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Funding: 教育部高等学校博士学科点专项科研基金（No.No.20106101110012）
Opened online:23 January 2013
Accepted by: none
Citation: YANG Ganglong,MA Tianran,LI Zheng.Isolation and Characterization of Total N-linked Glycans from Glycoproteins by Ultrafiltration Units and Mass Spectrometry[OL]. [23 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4514464
 
 
Glycosylation is one of the most common post-translational modifications in proteins. Current methods for glycan analysis are generally based on multiple preparation processes to separate glycans. However, glycans are continuously lost and the difficulty for accurate quantitative analysis is increased in the procedure. Here, a filter aided sample preparation-based total N-linked glycans from the glycoproteins isolation method (N-glycan-FASP-T) was developed using ultrafiltration units according to the molecular weight difference among the glycans, the impurities and proteins. Results: The isolated glycans were characterized and confirmed by the MALDI-TOF/TOF-MS. A total of 23 distinctive N-linked glycans were characterized from human sera.
Keywords:Biochemistry and molecular biology; N-linked glycans; Filter aided sample preparation-based total N-linked glycans from glycoproteins isolation method; Ultrafiltration unit; Glycoproteins; MALDI-TOF/TOF-MS
 
 
 

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