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Overexpression of Thioredoxin Reductase Gene TRR1 in Saccharomyces cerevisiae
CONG Wenrong,CHEN Jingyu *
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
*Correspondence author
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Funding: Specialized Research Fund for the Doctoral Program of Higher Education （No.No. 20090008120028）, National Natural Science Foundation of China （No.No. 31101237）
Opened online:25 February 2013
Accepted by: none
Citation: CONG Wenrong,CHEN Jingyu.Overexpression of Thioredoxin Reductase Gene TRR1 in Saccharomyces cerevisiae[OL]. [25 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4520837
 
 
Thioredoxin reductase 1 (Trr1p) is one of the most important parts in the antioxidative system of Saccharomyces cerevisiae. It plays a significant role in maintaining cells' redox state. In this study, according to the oxidative stress tolerance mechanism, thioredoxin reductase gene TRR1 was cloned onto vector pYES2 to generate recombinant plasmid pYES2-trr1, by which TRR1 gene was overexpressed in S. cerevisiae. The effects of TRR1 gene overexpression were investigated under oxidative stress induced by H2O2 or menadione. The results showed that TRR1 gene overexpression could apparently enhance antioxidative ability of S. cerevisiae.
Keywords:fermentation engineering; Saccharomyces cerevisiae; thioredoxin reductase; antioxidation
 
 
 

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