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Drosha-DGCR8 complex and Dicer recognize their RNA substrates in different manners during miRNA processing
FENG Yong 1 * #,CHEN Lang 2,HOU Wei 2
1.Wuhan University School of Basic Medical Sciences, WuHan 430070
2.Wuhan University School of Basic Medical Sciences
*Correspondence author
#Submitted by
Subject:
Funding: ph.D. Program Foundation of Chinese Ministry of Education （No.No. 20090141120048 ）, National Natural Science Foundation of China（No.No. 30972754）
Opened online: 6 March 2013
Accepted by: none
Citation: FENG Yong,CHEN Lang,HOU Wei.Drosha-DGCR8 complex and Dicer recognize their RNA substrates in different manners during miRNA processing[OL]. [ 6 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4523869
 
 
Drosha/DGCR8 complex cleaves primary microRNAs (pri-miRNAs) to yield ~ 65 nucleotide (nt) precursor microRNAs (pre-miRNAs). Dicer cleaves pre-miRNAs to yield ~ 22 nt miRNA/miRNA* duplexes. Interactions of Drosha/DGCR8 and Dicer with their substrates are incompletely understood. By in vitro Drosha and Dicer assays, we proposed models that human Drosha/DGCR8 and Dicer recognize their substrates in different manners. The flanking sequences and the terminal loop structure of a pri-miRNA are critical for Drosha/DGCR8 processing pri-miRNAs. The terminal loop of a pre-miRNA is not essential for hDicer processing. The end side and the stem of the pre-miRNA contribute to the recognizing and binding with Dicer. This study improved our understanding on RNase III functional mechanism and miRNA processing.
Keywords:microRNA; Drosha; Dicer; RNA processing
 
 
 

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