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| Most fungi have two different conidiation patterns: normal conidiation and microcycle conidiation under different culture conditions, including the nutrients in the medium. However, the mechanism of conidiation pattern shift response to culture conditions is poorly understood. In this study, Metarhizium acridum conducting microcycle conidiation on sucrose yeast extract agar medium (SYA) shifted to normal conidiation when supplemented with sucrose, nitrate, or phosphate. The M. acridum transcripts on SYA, SYA supplemented with sucrose, nitrate or phosphate, and 1/4SDAY were sequenced by Solexa/Illumina deep-sequencing technology. The results showed that 6,931 genes involved in 243 biochemical pathways were either up- or downregulated in response to different nutrients. Comparative analysis demonstrated that 1,859 genes specifically upregulated at the microcycle conidiation stage were selected. One hundred and twelve, 292, or 216 genes might be associated with microcycle conidiation after adding sucrose, nitrate, or phosphates to SYA medium, respectively. Four hundred and seventy genes were specifically upregulated and might be related to normal conidiation. KEGG pathway analysis indicated that these genes were mainly associated with the cell cycle, cell proliferation, metabolism, protein transport and signal transduction, and the mitogen-activated protein kinase signaling and catabolism pathway. Changing sucrose, nitrate or phosphate, led to a shift between microcycle and normal conidiation by perturbing the cell cycle and metabolism of M. acridum. This research provides essential information about the molecular mechanism of conidiation pattern shift induced by single nutrients. |
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| Keywords:Metarhizium acridum; conidiation pattern shift; pathway analysis; Gene expression profiling |
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