Engineering Bacillus subtilis for acetoin production from glucose and xylose mixtures

CHEN Tao; LIU Weixi; FU Jing; ZHANG BO

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Engineering Bacillus subtilis for acetoin production from glucose and xylose mixtures

CHEN Tao 1 * #,LIU Weixi 2,FU Jing 2,ZHANG BO 2

1.Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072

2.Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, People’s Republic of China

*Correspondence author

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Funding: Natural Science Foundation of Tianjin （No.12JCYBJC12900）, the Research Fund for the Doctoral Program of Higher Education（No.20100032120014）, the National High Technology Research and Development Program of China（No.2012AA023102B, 2012AA022103B）, This work was supported by the National 973 Project （No.2012CB725203, 2011CBA00804）, National Natural Science Foundation of China（No.NSFC-21176182）

Opened online:28 April 2013

Accepted by: none

Citation: CHEN Tao,LIU Weixi,FU Jing.Engineering Bacillus subtilis for acetoin production from glucose and xylose mixtures[OL]. [28 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4539683

As a vital flavor compound, acetoin is extensively used in dairy products and drinks industry. In this study, Bacillus subtilis was engineered to metabolize glucose and xylose as substrates for acetoin production. Initially, gene araE from B. subtilis, encoding the xylose transport protein AraE, was placed under the control of the constitutive promoter P43 for over-expression. Batch cultures showed that 10 g/L xylose was depleted completely in 32 hours. Subsequently, genes xylA and xylB from Escherichia coli, encoding xylose isomerase and xylulokinase respectively, were introduced into B. subtilis, and the recombinant turned out to assimilate glucose and xylose without preference. In shake-flask fermentations, 5.5 g/L acetoin with a yield of 0.70 mol (molosugar)-1 was obtained by the optimum strain BSUL13 under microaerobic conditions, which offered a metabolic engineering strategy on engineering microbe as cell factory for the production of high-valued chemicals from renewable resource.

Keywords:acetoin; Bacillus subtilis; carbon catabolite repression; metabolic engineering; xylose

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