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miR-126 Functions as a Tumor Suppressor in Osteosarcoma by Targeting Sox2
YANG Chenglin,HOU Chunyin,ZHANG Hepeng,WANG Dewei,MA Yan,ZHANG Yunqi,XU Xiaoyan,BI Zhenggang,GENG Shuo * #
Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China
*Correspondence author
#Submitted by
Subject:
Funding: Research Fund of the First Affiliated Hospital of Harbin Medical University （No.2013LX01）, National Natural Science Foundation of China （No.81271984）, Natural Science Foundation for Returnees of Heilongjiang Province of China （No.LC2012C11）, Research Fund for the Doctoral Program of Higher Education of China （No.20122307120036）, Research Funds for the Returnees of Education Department of Heilongjiang Province of China （No.1253HQ003）
Opened online:20 December 2013
Accepted by: none
Citation: YANG Chenglin,HOU Chunyin,ZHANG Hepeng.miR-126 Functions as a Tumor Suppressor in Osteosarcoma by Targeting Sox2[OL]. [20 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4575255
 
 
Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults, the early symptoms and signs of which are non-specific. The discovery of microRNAs (miRNAs) provides a new avenue for the early diagnosis and treatment of OS. MiR-126 has been reported to be highly expressed in vascularized tissues, and is recently widely studied in cancers. Herein, we explored the expression and significance of miR-126 in OS. Using TaqMan RT-PCR analysis, we analyzed the expression of miR-126 in 32 paired OS tumor tissues and 4 OS cell lines and found that miR-126 was consistently under-expressed in OS tissues and cell lines compared with normal bone tissues and normal osteoblast cells (NHOst), respectively. As miR-126 is significantly decreased in OS tissues and cell lines, we sought to compensate for its loss through exogenous transfection into MG-63 cells with a miR-126 mimic. Ectopic expression of miR-126 inhibited cell proliferation, migration and invasion, and induced apoptosis of MG-63 cells. Moreover, bioinformatic prediction suggested that the sex-determining region Y-box 2 (Sox2) is a target gene of miR-126. Using mRNA and protein expression analysis, luciferase assays and rescue assays, we demonstrate that restored expression of Sox2 dampened miR-126-mediated suppression of tumor progression, which suggests the important role of miR-126/Sox2 interaction in tumor progression. Taken together, our data indicate that miR-126 functions as a tumor suppressor in OS, which exerts its activity by suppressing the expression of Sox2.
Keywords:Surgery; Osteosarcoma; miR-126; tumor suppressor; sex-determining region Y-box 2
 
 
 

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