Analysis of monosaccharides in the triterpene glycosides from sea cucumbers by high performance liquid chromatographic (HPLC)

GAO Xiaomeng; DONG Ping; XUE Changhu; XU Jie; WANG Yuming

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Analysis of monosaccharides in the triterpene glycosides from sea cucumbers by high performance liquid chromatographic (HPLC)

GAO Xiaomeng #,DONG Ping *,XUE Changhu,XU Jie,WANG Yuming

College of Food Science and Technology, Ocean University of China, Qingdao 266003

*Correspondence author

#Submitted by

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Funding: The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry（No.NO. 05009）, Higher school specialized research fund for the doctoral program funding issue（No.NO. 20100132120018）

Opened online:30 December 2013

Accepted by: none

Citation: GAO Xiaomeng,DONG Ping,XUE Changhu.Analysis of monosaccharides in the triterpene glycosides from sea cucumbers by high performance liquid chromatographic (HPLC)[OL]. [30 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4577661

To fast and effectively analyze the sugar chain of triterpene glycosides from sea cucumber, a method was developed using a reverse-phase high-performance liquid chromatography (HPLC) with 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. An optimized chromatographic condition was established in the present study. The calibration curves of monosaccharides (D-glucose, D-3-O-methylgucose, D-xylose and D-quinovose) were linear within the range of 0.05 - 1 mmol/L (R2 > 0.996). The detection limits of these four monosaccharides were below 5 pmol. Monosaccharides of holothurin A and holothurin B were analyzed by this method. The result showed it was simple, rapid, stable, and less sample consumed than previous methods.

Keywords:sea cucumber; triterpene glycoside; monosaccharide determination; 1-phenyl-3-methyl-5-pyrazolone (PMP); derivatization; high performance liquid chromatographic (HPLC).

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