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| Leaf trichome plays an important role in protecting plants against insect herbivores, loss of water through transpiration and UV irradiation. In our present study, SSSL-W24, a single segment substitution line (SSSL) containing only one chromosome segment of the glabrous japonica cultivar Star bonnet 99 in the genetic background of the pubescent indica cultivar HJX74 showed glabrous leaves and hulls. Genetic analysis of the F2 generation, derived from a cross between SSSL-W24 and HJX74, showed that these traits are controlled by the recessive gene gl-1, which was mapped to the short arm of chromosome 5. Fine mapping and high-resolution linkage analysis using 1585 F3 plants and markers ?anking gl-1 were carried out, and the gene was localized to a 35.9 kb region that contains seven annotated genes according to the genome sequence of japonica Nipponbare. Positional cloning of this gene will assist selection of hybrid rice, facilitate the mechanization of agriculture and increase the warehousing capacity of rice. |
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| Keywords:Crop genetic breeding; Rice; Trichome; Single segment substitution line; Fine mapping; Glabrous gene; |
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