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Pre- and post-treatment with edaravone protects CA1 hippocampus and enhances neurogenesis in the subgranular zone of dentate gyrus after transient global cerebral ischemia in rats
LEI Shan 1,ZHANG Pengbo 2 *,LI Weisong 2,GAO Ming 2,HE Xijing 3,ZHENG Juan 2,LI Xu 2,WANG Ning 2,WANG Xiao 2,ZHANG Junfeng 4,QI Cunfang 4,LV Haixia 4,CHEN Xinlin 4,LIU Yong 4
1. Department of Anesthesiology, Second Affiliated Hospital of Xi’an Jiaotong University School of Medicine, 157# West 5 road, Xi’an 710004
2.Department of Anesthesiology, Second Affiliated Hospital of Xi’an Jiaotong University School of Medicine, 157# West 5 road, Xi’an 710004
3.Department of Orthopedics, Second Affiliated Hospital of Xi’an Jiaotong University School of Medicine, 157# West 5 road, Xi’an 710004
4.Institute of Neurobiology, National Key Academic Subject of Physiology of Xi’an Jiaotong University School of Medicine, 76# Yanta West Road, Xi’an 710061
*Correspondence author
#Submitted by
Subject:
Funding: the Program for New Century Excellent Talents in University of China (No.NCET-08-0436), the National Natural Science Foundation of China (No.No. 8107107), The Specialized Research Fund for the Doctoral Program in Higher School of China (No.20100201110051)
Opened online:16 January 2014
Accepted by: none
Citation: LEI Shan,ZHANG Pengbo,LI Weisong.Pre- and post-treatment with edaravone protects CA1 hippocampus and enhances neurogenesis in the subgranular zone of dentate gyrus after transient global cerebral ischemia in rats[OL]. [16 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4581616
 
 
Edaravone is clinically used for treatment of patients with acute cerebral infarction. However, the effect of edaravone on neurogenesis in the hippocampus following ischemia remains unknown. In the present study, we explored whether pre- and post- treatment of edaravone had any effect on neural stem/progenitor cells (NSPCs) in the subgranular zone (SGZ) of hippocampus in a rat model of transient global cerebral ischemia. Male Sprague-Dawley rats were divided into 3 groups, sham-operated (n = 15), control (n = 15), and edaravone-treated (n = 15) groups. Newly-generated cells were labeled by 5-bromo-2-deoxyuridine (BrdU). Immunohistochemistry was used to detect neurogenesis in the SGZ at 7, 14 and 21 d following ischemia. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) was used to detect cell apoptosis. Our results indicate that pre- and post-treatment with edaravone enhances neurogenesis and protects NSPCs from apoptosis in the hippocampus.?
Keywords:neurobiology; neurogenesis; cerebral ischemia; neural stem/progenitor cells; neuroprotection; edaravone
 
 
 

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