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| In this study, an antioxidant gene was isolated and cloned from the widespread plant Capsella bursa-pastoris. The antioxidant gene was homologous highly to the Arabidopsis ATPER1 gene, and was named as CbPER1. We deduced the amino acid sequence of CbPER1 protein, and used it to conduct a phylogenetic tree combining with 1-Cys Prxs (1-cysteine peroxiredoxins) from other plants for estimating the relationships. Using RT-PCR, we analyzed the expression of CbPER1 in different organs and embryonic stages. CbPER1 was extensively expressed in seeds, especially middle and later seeds. Besides, CbPER1 rarely responded to heat stress, cold stress, and ABA treatment in young leaves. Similarly, CbPER1 was not induced to express in young flowers when C. bursa-pastoris plants were treated with ABA. CbPER1 showed down-regulation in dormant embryos after GA treatment. Furthermore, the transcriptional level of CbPER1 in C. bursa-pastoris was compared to that of the Arabidopsis AtPER1, and both shared similar gene expression profiles. Our findings suggest that the CbPER1 genes play significant roles in seed maturation, dormancy, and germination, not in the response to abiotic stresses in C. bursa-pastoris. |
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| Keywords:Peroxiredoxin gene ;Gene cloning; Gene expression |
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