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| Reduning injection, a traditional Chinese medicine injection, has multiple functions such as clearing heat, dispelling wind, as well as detoxification. However, there are a few reports of severe anaphylaxis during the clinical application of reduning. The aim of this study is to determine the sensitization to reduning in guinea pigs and the underlying cause of the anaphylactic reaction. The compositions and Tween-80 in reduning were determined before and after ultrafiltration. Egg albumin, ultrafiltered reduning, unfiltered reduning, Tween-80 and nine compositions in reduning were selected to sensitize and stimulate the animals. Changes in the levels of plasma 5-hydroxytryptamine were used to analyze the effect of ultrafiltration on the sensitization effect of reduning injection. We detected a significant decrease in Tween-80 content but a less significant decrease in the other components of the injection following ultrafiltration. Unfiltered reduning injection, Tween-80, and chlorogenic acid caused remarkable sensitization on guinea pigs while the filtered reduning injection resulted in a significantly lower degree of sensitization. These results suggest that ultrafiltration significantly reduced the sensitizing effect of reduning injection, which is likely due to Tween-80 content. Additionally, the form of chlorogenic acid within the complex solution mixture may also affect the sensitizing effect of chlorogenic acid. |
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| Keywords:Reduning injection, Ultrafiltration, Tween-80, Chlorogenic acid, Sensitization |
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