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| The EST-SSR markers of Paecilomyces genus was established with the primers designed according to ESTs pools of Paecilomyces militaris. The markers had well transferability to Paecilomyces hepialid. Genetic diversity and structure were also evaluated within and among 11 populations of P. militaris. Nine primer groups were screened, and a total of 179 DNA fragments were amplified, all of which were polymorphic, averaging 19.9 polymorphic bands per primer. At the species level, the percentage of polymorphic bands was 100.00%, and 28.08% within the 11 populations. The observed number of alleles (Na) was 2.0000 and the effective number of alleles (Ne) was 1.3278; Nei's gene diversity (H) was 0.2128; Shannon's information index (I) was 0.3452. At the population level, Na = 1.3017, Ne = 1.1677, H = 0.0918, and I = 0.1546. Total gene diversity was 0.2027(Ht), the gene diversity within population was 0.0918(Hs), the coefficient of gene differentiation was 0.5472(Gst), and estimated gene flow was 0.4138(Nm). These results indicated the genetic differentiation was relatively higher among populations of P. militaris. The genetic structure had correlation with the geographical source, but not so obvious among the populations in Yunnan. The Paecilomyces sp. isolated from living Hepialus yunnanensis was proved to be Paecilomyces hepialid. The EST-SSR markers were an informative and useful method for assaying genetic diversity and authenticating species of Paecilomyces. The EST-SSR markers would provide for insight into population genetics of Paecilomyces species and help us select genotypes with high adaptability and high content of useful chemical components. |
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| Keywords:Paecilomyces militaris; Paecilomyces hepialid; Genetic Structure; EST-SSR |
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