Derivative method for both phase extraction and morphology identification of typical blood cells under interference microscopy

Xu Yuanyuan; Wang Yawei; Ji Ying; Jin Weifeng

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Derivative method for both phase extraction and morphology identification of typical blood cells under interference microscopy

Xu Yuanyuan 1,Wang Yawei 1 *,Ji Ying 2,Jin Weifeng 2

1.School of Mechanical Engineering,Jiangsu University,Zhenjiang 212013

2.Faculty of Science,Jiangsu University,Zhenjiang 212013

*Correspondence author

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Funding: Jiangsu Province colleges and universities Major Project（No.No.09KJA140001）, Doctoral Program Joint Fund of colleges and universities Specialized Research（No.No.20113227110018）, National Natural Science Foundation of China（No.Nos.11374130,11302086）

Opened online:12 December 2014

Accepted by: none

Citation: Xu Yuanyuan,Wang Yawei,Ji Ying.Derivative method for both phase extraction and morphology identification of typical blood cells under interference microscopy[OL]. [12 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4622231

We present a derivative method for phase information extraction in off-axis interference microscopy. By numerically calculating the interference term, the first-order and second-order derivatives of the interferogram, one can directly obtain the phase image. We illustrate the proposed method with both the theory analysis and some QPI simulations under typical blood cells' models. Compared with other typical phase extraction methods, this method has the ability of fast data processing. Furthermore, the first-order derivative of the phase is introduced to analyze the cell morphology, and a monocyte model and a red blood cell model are discussed as the examples to demonstrate the method. Results show that the first-order derivative of the phase can be used as a tool for the identification of blood cells.

Keywords:Interference microscopy; blood cells; phase extraction;derivative, simulation

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