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| he reducing barrier, using the compacted sewage sludge to be the liner for the tailings, made the liner have both encapsulating and eliminating functions. The microbial functional diversity ,heavy metal concentration, sulfate reducing bacteria (SRB) and total bacteria (TB) quantity in the compacted sludge with different seepage conditions (the deionized water(DW), the pH2.1 sulfuric acid and the simulate acid mine drainage(AMD)) were studied using Biolog and the Most Probable Number technique(MPN) testing system. The results showed that the average well color development (AWCD), the Shannon index (H), the Evenness index (E), the number of positive Ecoplate wells (S), the total bacteria (TB), and the sulfate reducing bacteria (SRB) quantity differed between different seepage conditions. There was no correlation between SRB quantity, Total bacteria quantity (TB) and the microbial community level functional diversity data. There was correlation between Cu concentration and Fe, Ni, Zn; between Fe and Ni. However, there was no correlation between Cd and other heavy metals. There was highly negative correlation between Cu, Fe, Ni concentration and the microbial community level functional diversity data. SRB quantity had correlation with Cd and Cu concentration. And TB quantity had correlation with Cu, Fe and Zn concentration. |
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| Keywords:Waste Disposal; Acid mine drainage(AMD); Compacted sewage sludge; Heavy metals; Microbial functional diversity; Seepage |
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