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| Compacted sewage sludge barrier for tailings, known as reducing barrier, was innovatively double- functioned liner with its capsulation and elimination to Acid Mine Drainage (AMD) produced by tailings. The research had provided insights regarding the bacterial community structure diversity and dynamics over 75 days in the compacted sewage sludge specimens under 3 different seepage conditions (deionized water (DW), pH 2.1 sulfuric acid water (SA) and the synthetic AMD), by PCR-DGGE technique. The results indicated that at the start point (SP) of seepage, the 41st day (T1) of seepage or at the 75th day (T2) of seepage, under different seepage conditions (DW, SA and AMD), Clostridiales ,Bacillaceae and Carnobacteriaceae dominated in the compacted sewage sludge specimen with different relative abundance ranged from 46.26 % to 10.25 %. Redundancy analysis (RDA) on the influences between environmental factors (pH and heavy metals potential mobility in the compacted sludge specimens) and microbial community structure diversity showed the bacteria such as Carnobacteriaceae, Bacillaceae, Clostridiales Family XI. Incertae Sedis, Clostridiaceae, Lactobacillaceae, Peptoniphilaceae, Ruminococcaceae, Moraxellaceae, Caulobacteraceae, Corynebacteriaceae had positive relation to Zn potential mobility and pH; Carnobacteriaceae, Bacillaceae, Clostridiales Family XI. Incertae Sedis, Clostridiaceae, Micrococcineae, Moraxellaceae, uncertain family bacteria had positive relation with Fe potential mobility; Carnobacteriaceae, Clostridiaceae, Corynebacteriaceae had positive relation to Cd and Ni potential mobility; Ruminococcaceae had positive relation to Cu potential mobility. |
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| Keywords:Environmental microbilogy;Compacted sewage sludge; Microbial community structure diversity; PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE); Reducing barrier;Acid mine drainage (AMD) |
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