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| In this paper the effects of dietary daidzein alone or in combination with lactulose on humoral immunity, liver oxidant status and colon microbiota of barrows (Landrace×Duroc) challenged with Escherichia coli lipopolysaccharide (LPS). A total of 36 barrows (age, 40 d; initial BW, 9.35±0.17 kg) were randomly allotted into 6 pens (6 pigs/pen; 2 pens/treatment). Dietary treatments were: 1) control (C, n=12, fed an isoflavones-free basic diet), 2) control+daidzein (D, n=12, fed an isoflavones-free basic diet with 50mg/kg of daidzein supplementation), and 3) control+daidzein+lactulose group (D+L, n=12, fed an isoflavones-free basic diet with 1% of lactulose and 50mg/kg of daidzein supplementation). After 23 days, 6 of pigs from each treatment were randomly selected and injected with LPS. After 24 h post injection, dietary daidzein was found significantly increased the peripheral blood lymphocyte proliferation response to both and PHA and LPS stimulation, and daidzein combination with lactulose supplementation had higher response. After 48 h post injection, pigs were killed. In the D and D+L groups, plasma IL-6 level was lower than C group, while cortisol concentration was higher. Furthermore, dietary daidzein or combination with lactulose may improve the profiles of colonic microflora, and maintains the bacteria populations, including hydrogen-utilizing bacteria after LPS challenge. Moreover, by adding lactulose can alleviates the liver oxidative stress caused by LPS challenge in barrows treated with daidzein. |
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| Keywords: Animal nutrition;Lactulose; Equol; Humoral Immunity; Anti-oxidant; Colon Microbiota |
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