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| Akirin as a conserved nuclear protein functions in parallel with the NF-κB of the immune deficiency (IMD) pathway and the Toll-like receptor (TLR) pathway. In this study, the full-length cDNAs of Akirin and 14-3-3 in Marsupenaeus japonicus, named as MjAki and Mj14-3-3 were identified separately. MjAki was mainly expressed in hemocytes, heart and intestine and Mj14-3-3 was detected in all six tested tissues including hemocytes, heart, hepatopancreas, gills, stomach, and intestine. The expression of MjAki was upregulated in the hemocytes of shrimp challenged with Vibrio anguillarum and white spot syndrome virus (WSSV), Mj14-3-3 was first decreased and then increased in the shrimp challenged with V. anguillarum and no obviously change in the shrimp challenged with WSSV. The expression of some AMPs was obviously suppressed by knockdown of MjAki and Mj14-3-3 with RNA interference. The interaction of MjAki with Mj14-3-3, dorsal, relish was confirmed in vitro. We proposed that MjAki combined with Mj14-3-3, dorsal and relish, acted as an active nuclear factor of NF-κB dependent pathway in innate immunity of M. japonicus. |
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| Keywords:Akirin; 14-3-3; NF-κB; Dorsal; Relish; Marsupenaeus japonicus; innate immunity |
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