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| Outbreaks of skin and internal neoplasia in commercial Hyline layer flocks occurred in eastern China during the years of 2009 to 2010. Anemia, reduction in egg production and high mortality (up to 20%) were common characters of affected flocks. Most of tumors were present in the skin of trunk, digitus, joint, face and wing, and in the liver, spleen, heart, small intestine and pancreas at 26-week of age. The tumors looked exactly like blood blisters in various sizes. Microscopically, they were defined as hemangiomas of typical capillary and cavernous form, characterized by greatly distended blood spaces with thin walls composed of endothelial cells. Myelocytomas, fibrosarcomas and myxoma were observed in few hemangiomas cases. Three strains of avian leukosis virus subgroup J (ALV-J) were isolated from DF-1 culture (WS0703, WS0704 and WS0705). Sequences analysis of the gp85 gene of the three strains revealed that these viruses are closely related to HPRS-103 (98.3%), a prototype of ALV-J. There was no evidence of Marek's disease virus (MDV) infection. Proviral DNA of avian leukosis virus subgroup A/B (ALV-A/B) and Reticuloendotheliosis virus (REV) was not detected by polymerase chain reaction (PCR) either in tumors or in DF-1 cultures. The report demonstrated that hamangiomas presentation associated with not only multipotential oncogenicity of ALV-J but also the line of chicken. The pathogenesis need to be further investigated.????? |
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| Keywords:Veterinaary;Hemangiomas; ALV-J; Hyline layer flocks; gp85 gene; |
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