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| Recently, urine-derived stem cells (USCs) have shown application potential for wound healing based on cell therapy strategy. However, the mechanism remains unclear. As it has been reported the interactions between stem cells and recipient cells in wound sites can stimulate wound healing through paracrine effects, we hypothesized that the wound healing ability of USCs might be related to paracrine effects between USCs and recipient cells involved in wound healing, mainly including endothelial cells and fibroblasts. In addition, our previous studies demonstrated that bioactive silicate materials could stimulate paracrine effects between bone marrow stem cells and endothelial cells as well as fibroblast and endothelial cells, which finally enhanced vascularization in bone regeneration. Therefore, in this study, we aimed to investigate the paracrine effects between USCs, human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and to study the effects of bioglass (BG) on these paracrine effects in order to explore the application potential of BG combining USCs on wound healing. Results show that BG ion extracts can not only affect behaviors of USCs but also stimulate paracrine effects between USCs and HUVECs, USCs/HDFs and USCs/HUVEC-HDF co-cultures, which results in stimulated vascularization of HUVECs, promoted ECM protein production and myofibroblast differentiation of fibroblasts either in indirect contact co-cultures or in USC-HUVEC direct contact co-cultures. Taken together, USCs possess application potential in wound healing based on cell therapy due to its strong effects on fibroblast and endothelial cells. More importantly, BG can be used to promote wound healing ability of USCs in tissue engineering therapy though enhancing the interactions between USCs and other types of cells. |
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| Keywords:Urine-derived stem cells; cell-cell interactions; paracrine effects; vascularization; wound healing |
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