Negative Correlation of RNA Epigenetic Modifications of N6-methyladenosine and 5-methylcytosine with Aging

JIANG Han-Peng; XIONG Jun; HUANG Wei; LAN Meng-Dan; ZENG Huan; FENG Yu-Qi; YUAN Bi-Feng

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Negative Correlation of RNA Epigenetic Modifications of N6-methyladenosine and 5-methylcytosine with Aging

JIANG Han-Peng,XIONG Jun,HUANG Wei,LAN Meng-Dan,ZENG Huan,FENG Yu-Qi,YUAN Bi-Feng * #

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China

*Correspondence author

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Funding: National Natural Science Foundation of China（No.21522507, 21475098）, National Basic Research Program of China (973 Program)（No.2012CB720601, 2013CB910702）, Ph.D. Programs Foundation of Ministry of Education of China（No.20120141120037）

Opened online:27 May 2016

Accepted by: none

Citation: JIANG Han-Peng,XIONG Jun,HUANG Wei.Negative Correlation of RNA Epigenetic Modifications of N6-methyladenosine and 5-methylcytosine with Aging[OL]. [27 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4691753

Aging is characterized by progressive decline in physiology and function of adult tissues. Previous studies demonstrated DNA methylation is related to aging. In addition to DNA methylation, reversible RNA modification has recently been proposed to represent another realm for biological regulation in the form of "RNA epigenetics". However, the correlation of emerging RNA epigenetic modifications in aging process is still a mystery. Here we developed an on-line trapping/capillary hydrophilic-interaction liquid chromatography/electrospray ionization-mass spectrometry method for ultra-sensitive and simultaneous quantification of RNA epigenetic modifications of N6-methyladenosine (m6A) and 5-methylcytosine (5-mC) from human blood. We analyzed the contents of m6A and 5-mC in RNA of blood from 260 healthy persons aged from 0 to 88 years old. The results showed that both m6A and 5-mC contents were significantly decreased and highly correlated with aging process. We then established RNA epigenetic modifications age models according to m6A and 5-mC contents with an mean absolute deviation of 6.7 years for m6A and 8.0 years for 5-mC. This study demonstrated RNA epigenetic modifications of m6A and 5-mC represents strong and reproducible marks of chronological age, which could be potentially applied in health assessment and prevention of diseases.

Keywords:Analytical chemistry; N6-methyladenosine, 5-methylcytosine, aging, hydrophilic-interaction liquid chromatography, capillary monolithic column

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