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| Objective: To explore a new model for the study of spinal cord injury, and to prove the possibility of this model. Methods: The Thy1( thymus cell antigen 1, theta)-YFPH transgenic mice and DiI tracing were employed to observe the distribution of corticospinal tract (CST). And lateral spinal cord hemisection injury model was made using the Thy1-YFPH mice. Results: Pyramidal neurons in cerebral cortex layer Ⅴ was specifically labeled and projected to pyramidal decussation in the medulla. In the spinal cord, the YFP labeled projections were distributed at the same locations as dorsal CST (dCST), dorsolateral CST (dlCST) and ventral CST (vCST). YFP positive axons were mostly colocalized with DiI traced dCST both in the spinal cord of uninjured Thy1-YFPH mice. Conclusion: The Thy1-YFPH mice could label the CST in the mouse spinal cord, and could be used as an easy and convenient model to monitor the degeneration, regeneration and remodeling of CST axons after spinal cord injury. |
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| Keywords:spinal cord injury; CST; Thy1-YFPH transgenic mice |
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