A colorimetric and fluorescent probe for rapid determination of copper in water and living cells

Nie Hailiang; Zhang Xiaoling

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A colorimetric and fluorescent probe for rapid determination of copper in water and living cells

Nie Hailiang 1,Zhang Xiaoling 2 * #

1.Beijing Institute of Technology, Chemistry and chemical engineering, Beijing 100081

2. Beijing Institute of Technology, Chemistry and chemical engineering, Beijing 100081

*Correspondence author

#Submitted by

Subject:

Funding: Doctoral Program of Higher Education of China （No.No. 20121101110049）

Opened online: 5 May 2017

Accepted by: none

Citation: Nie Hailiang,Zhang Xiaoling.A colorimetric and fluorescent probe for rapid determination of copper in water and living cells[OL]. [ 5 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4728954

Detection of copper ions (Cu2+) is of great significance in protecting the environment and human health. In the present work, we developed a new colorimetric and fluorescent probe for Cu2+ determination with high selectivity and rapid-response rate. Upon Cu2+ addition, the probe underwent an 86 nm blue-shift in absorption peak wavelength as well as a significant quenching in fluorescence emission. The fluorescence intensity at 575 nm showed a good linear relationship (R2 = 0.999) with the Cu2+ concentration in the range of 0 μM ~ 12 μM. Using the probe as an analytical tool, the detection of Cu2 + can be done within 5 s, whose process was not subject to interference from other metal ions and pH changes. Moreover, the probe can be used for Cu2+ assay in both real water sample and living cells.

Keywords:Colorimetric and fluorescent probe; Fluorescence imaging.; Cu2+

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