Patterned Photonic Nitrocellulose Membrane for   Bio-detection Based on Coffee-Ring Effect

Litianyi Tang; Hong Liu

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Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect

Litianyi Tang,Hong Liu *

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

*Correspondence author

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Funding: none

Opened online:26 March 2018

Accepted by: none

Citation: Litianyi Tang,Hong Liu.Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect[OL]. [26 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743982

In this work, we report a method for the fabrication of nitrocellulose (NC) membrane with photonic crystal (PC) pattern for bio-detection. The membrane is prepared by imprinting the corresponding PC pattern into it through thermal Nano-imprint method. Aptamer beacon marked with fluorescein is used for detection of target molecules. Only one droplet (2-3μl) containing the aptamer-target complexes is dripped onto the nitrocellulose membrane. After evaporation (15-20min), the droplet is completely dried and the complexes form a coffee ring on the membrane. The ring keep growing with the increase of complexes concentration .As the aptamer beacon is marked with fluorescein and PC pattern has a fluorescence enhancement effect, the fluorescent signal on the coffee ring for detection is considerable improved. Fluorescent detection of adenosine triphosphate (ATP) is used for testing the application of this method. Owing to fluorescence enhancement effect of PC, the fluorescent signal for detection is improved and the limit of detection is 80.2μM for ATP. Therefore, we believe this method is promising for the fabrication of patterned photonic nitrocellulose membrane for bio-detection and point-of-care testing.

Keywords:Analytical Chemistry; Photonic crystal；Fluorescence enhancement；Coffee-ring；Nano-imprint；POCT

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