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Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation
Yang Jingru 1,He Huahong 2,He Huafeng 2,Wang Huaqian 1 *
1.School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006;Guangzhou Institute For Drug Control, Guangzhou 510160;School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006;School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006
2.
*Correspondence author
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Funding: the National Natural Science Foundation of China （No.No. 81501429)）, the Natural Science Foundation of Guangdong Province （No.No. S2012040007488）
Opened online: 6 April 2023
Accepted by: none
Citation: Yang Jingru,He Huahong,He Huafeng.Study on the renaturation process of epidermal growth factor-like protein and its therapeutic effect on neuroinflammation[OL]. [ 6 April 2023] http://en.paper.edu.cn/en_releasepaper/content/4759631
 
 
Epidermal growth factor (EGF) plays an important role in improving the inflammatory response. In this study, cholera toxin A2 subunit, cell-penetrating peptide trans-transcription activator (TAT) and EGF were fused and recombinant protein EGF-CTA2-TAT were expressed by Escherichia coli. Two methods of renaturation, affinity chromatography and dialysis, were selected to renature the protein. The renaturation efficiency of these two methods were compared. Activities of recombinant protein on promoting BALB/c 3T3 cells proliferation in vitro were also studied. After that, mice model of neuroinflammation was established by using the Lipopolysaccharide (LPS) intraperitoneal injection. The purified and renatured recombinant EGF-like protein was administered intranasally for neuroinflammation treatment. The Morris water maze (MWM) test was used to evaluate the therapeutic effects. Our results showed that recombinant EGF-like protein had a therapeutic effect on LPS-induced neuroinflammation in mice, and could remarkably improve the learning and memory ability of mice. The recombinant EGF-like protein renatured and purified by affinity chromatography in one step shows the advantages of simple process, high yield, excellent activity and low cost, providing a reference for mass production of such active proteins.
Keywords:EGF; affinity chromatography renaturation; dialysis renaturation; neuroinflammation
 
 
 

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