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The Study of the Role of Glu 101 in Maintaining the Proper Conformation of Euplotes Octocarinatus Centrin
Li Guoting 1,Wang Zhijun 1 *,Zhao Yaqin 2,Ren Liexiang 1,Yang Binsheng 1
1.Institute of Molecular Science, Shanxi University
2.Institute of Molecular Science- Shanxi University
*Correspondence author
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Funding: 国家自然科学基金（No.No.20371031）
Opened online:23 May 2006
Accepted by: none
Citation: Li Guoting,Wang Zhijun,Zhao Yaqin.The Study of the Role of Glu 101 in Maintaining the Proper Conformation of Euplotes Octocarinatus Centrin[OL]. [23 May 2006] http://en.paper.edu.cn/en_releasepaper/content/6761
 
 
Glu is highly conserved as the first amino acid of E-helix of the EF-hand protein. In this paper, Glu 101, the first amino acid of the E-helix of the third EF-hand motif in EoCen was mutated to be Lys by the method of site direct mutation. TNS and Tb3+ were used as probes in the study of the effect of this mutation to the proper conformation of EoCen. Results indicate the mutation protein (E101K) displays an increased hydrophobic exposure surface and a different Tb3+ binding characteristic. It proves that the conformation of EoCen has been altered largely by this mutation. The general conditional binding constant of Tb3+ to the three loops of EF-hand sites I, II, III in E101K was calculated to be (5.64 ± 0.57) × 105 M-1.
Keywords:Centrin Tb3+ Fluorescence Spectra
 
 
 

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