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| A uracil grafted glassy carbon electrode (Ura/GCE) was fabricated. The electrode was characterized by x-ray photoelectron spectroscopy (XPS), cyclic voltammertry (CV) and differential pulse voltammetry (DPV) techniques. The electrochemical behaviors of Trp, Tyr and their derivatives, such as dopamine (DA), epinephrine (EP) and norepinephrine (NE) at Ura/GCE were investigated. All these bioactive species can be electrocatalytically oxidized at significantly reduced overpotentials and generate sensitive current signals at Ura/GCE. The linear range (detection limit, s/n=3) obtained for Tyr and Trp was 1.8 -12 0 礛 (0.8 礛) and 1.8 - 890 礛 (0.8 礛), respectively. In the coexistence of 1.0 mM AA (Ep(DPV)=-0.02 V), the linear range (detection limit, s/n=3) obtained for DA, EP, NE was 0.37 -36 礛 (0.2 礛), 0.38-13 礛 (0.2 礛), and 1.8 -84 礛 (0.8 礛), respectively. The Ura/GCE showed good electrocatalytic ability and anti-interference ability. The mechanism for Trp (Tyr) electrocatalytic oxidation was proposed.
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| Keywords:uracil, modified electrode, tryptophan, tyrosine, determination |
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