Development and validation of a sensitive reverse-phase HPLC method to determine intracellular accumulation of liposomal formulation of hydroxycamptothecin

Zhao Yongxing; Gao Jianqing; Qiao Hailing; Chen Hailiang; Liang Wenquan

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

Development and validation of a sensitive reverse-phase HPLC method to determine intracellular accumulation of liposomal formulation of hydroxycamptothecin

Zhao Yongxing 1 #,Gao Jianqing 1,Qiao Hailing 2,Chen Hailiang 1,Liang Wenquan 1

1.Zhejiang University

2.Zhengzhou University,Zhejiang University,Zhejiang University

*Correspondence author

#Submitted by

Subject:

Funding: 国家自然科学基金，教育部博士点基金（No.30572270，20050335044）

Opened online:16 December 2005

Accepted by: none

Citation: Zhao Yongxing,Gao Jianqing,Qiao Hailing.Development and validation of a sensitive reverse-phase HPLC method to determine intracellular accumulation of liposomal formulation of hydroxycamptothecin[OL]. [16 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4437

The intracellular accumulation of anti-cancer agents is strongly influence the efficiency of chemotherapy for cancer. In the present study, a simple, rapid, sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated to determine hydroxycamptothecin (HCPT) in Eca109 cells. Cells were treated with HCPT liposomes, and then lysed over ice by sonication. The HCPT in the samples were measured by RP-HPLC with a C18 column after a simple extraction with ethyl acetate. The mobile phase contained 0.1% triethylamine-phosphoric acid buffer (pH 3.0) and acetonitrile (75:25, v/v). The HCPT is monitored with fluorescence detector with excitation and emission wavelengths of 382 and 528 nm, respectively. The calibration curve was linear from 2 to 100ng/ml with correlation coefficient of 0.9999, while the limit of quantification is 2ng/ml. The mean extraction efficiency was 89.76%. The recovery of the assay was between 86.5 and 105.2%. The intra- and inter-day coefficients of variation were less than 10% (R.S.D.). Then the HCPT liposomes were prepared and the validated method was applied to intracellular accumulation study of both HCPT injection and HCPT liposomes in Eca109 cells. HCPT liposomes showed higher intracellular accumulation of HCPT in different incubation times compared with that of conventional HCPT injection.

Keywords:Hydroxycamptothecin; High-performance liquid chromatography; Cells; Determination; Liposomes

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	1482
Bookmarked	0
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers